Epitope binning-seq: A game-changer in antibody drug discovery

Antibody drug discovery is an extremely laborious and tedious process. Epitope Binning-seq, a novel method, introduces a groundbreaking approach by enabling simultaneous analysis of multiple antibodies. Using a fluorescently labeled reference antibody (rAb), this method employs flow cytometry to identify similar epitope-binding query antibodies (qAbs). Next-generation sequencing of fluorescence-negative cells then groups qAbs with epitopes akin to the rAb into epitope bins. This innovative technique holds immense potential in enhancing antibody drug discovery process.